Nocodazole | Microtubule Associated 抑制剂 | 现货供应 | 美国...

来自 : www.selleck.cn/products/nocoda 发布时间：2021-03-24

Cdc2 / cyclin B1 / MAD2 / Cdc20 ; PubMed: 21918689 PLoS One
E (upper part). Time-dependent changes in cyclin B1 and Cdc2 protein levels following nocodazole treatment. Cells were treated with nocodazole (250 nM) for the length of time as indicated, and whole cell lysates were prepared. An equal amount of protein lysates was electrophoretically separated on the 10% SDS-polyacrylamide gel, and transferred to nitrocellulose membrane. Western blots were detected using specific antibodies against cyclin B1, Cdc2 (CDK1), MAD2, and Cdc20 on an enhanced chemiluminescence (ECL) apparatus. Membrane was stripped for determining the levels of GAPDH as a loading control. E (lower part). The relative protein levels for cyclin B1, Cdc2, MAD2, and Cdc20 were calculated according to their densitometry readings, which were normalized according to the corresponding readings for the GAPDH protein bands. Each value is mean S.D. from three replicate determinations. *P 0.05, **P 0.01 versus vehicle-treated control.

Mol Cancer Ther, 2020, molcanther.0525.2020 PubMed: 33158997 ( click the link to review the publication ) Front Cell Dev Biol, 2020, 8:601972 ...Nocodazole (Oncodazole, R17934)是一种微管聚合的快速可逆抑制剂，也抑制Abl、Abl(E255K)和Abl(T315I)，对应的IC50值分别为0.21 μM，0.53 μM和0.64 μM。Nocodazole可提高CRISPR介导的同源重组效率，对增强精确的基因编辑效力具有加成作用。Nocodazole可诱导凋亡。Nocodazole